https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48064 2S may explain the dysregulation of microvascular tone associated with poor outcome following preterm birth. In adult vasculature, H₂S is predominantly produced by CSE. We hypothesized that vascular CSE activity contributes to microvascular tone regulation during circulatory transition. Methods: Preterm (GA62) and full‐term (GA69) guinea pig fetuses and neonates were studied. Microvascular blood flow was assessed by laser Doppler flowmetry. Thiosulfate, primary urinary metabolite of H₂S, was determined by high‐performance liquid chromatography. Real‐time H₂S production was assessed using a microrespiration system in fetal and postnatal (10, 24 hours) skin and heart samples. CSE contribution was investigated by inhibition via propargylglycine. Results: In preterm animals, postnatal H₂S production capacity in peripheral vasculature increased significantly and was significantly reduced by the inhibition of CSE. Urinary thiosulfate correlated with both microvascular blood flow and capacity of the vasculature to produce H₂S. H₂S produced via CSE did not correlate directly with microvascular blood flow. Conclusions: In preterm neonates, H₂S production increases during fetal‐to‐neonatal transition and CSE contribution to total H₂S increases postnatally. CSE‐dependent mechanisms may therefore underpin the increase in H₂S production over the first 72 hours of life in preterm human neonates, associated with both central and peripheral cardiovascular instability.]]> Wed 22 Feb 2023 13:57:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30962 Wed 11 Apr 2018 10:28:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36900 Wed 02 Mar 2022 14:24:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35009 A receptor subunits were measured by RT-PCR. Results: MBP immunostaining was increased in lobule IX of preterm males, and reduced in lobule X of preterm females when compared to their term counterparts. GAD67 staining was decreased in lobule IX and X of the preterm males, but only in lobule X of the preterm females compared to term cohorts for each sex. Internal granule cell layer width of lobule X was decreased in preterm cohorts of both sexes compared to terms. There were no differences between gestational age groups for NeuN staining, GAD67 and GAT1 protein expression as measured by western blotting, or GABA_A receptor subunits as measured by RT-PCR between preterm and term for either sex. Conclusions: The present findings suggest that components of the cerebellar GABAergic system of the ex-preterm cerebellum are disrupted. The higher expression of myelin in the preterm males may be due to a deficit in axonal pruning, whereas females have a deficit in myelination at 28 corrected days of age. Together these ongoing alterations may contribute to the neurodevelopmental and behavioural disorders observed in those born preterm.]]> Thu 30 May 2019 14:58:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29635 Sat 24 Mar 2018 07:41:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30294 Sat 24 Mar 2018 07:33:34 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44727 Mon 24 Oct 2022 08:12:40 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50958 Mon 14 Aug 2023 15:11:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48163 Fri 10 Mar 2023 16:25:45 AEDT ]]>